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ifn α protein concentration  (R&D Systems)


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    R&D Systems ifn α protein concentration
    Ifn α Protein Concentration, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ifn+%CE%B1+protein+concentration/Universal+Type+I+IFN+Protein/us12264324-127-8-18
    Average 95 stars, based on 60 article reviews
    ifn α protein concentration - by Bioz Stars, 2026-10
    95/100 stars

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    Protein Concentration:

    Article Title: Viral vector assay and vector
    Article Snippet: .. Diluted samples are analyzed in 96-well plate for IFN-α protein concentration by VerikineTM Human IFN-α ELISA kit (#41100 R & D Systems). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Viral vector assay and vector
    Article Snippet: .. Diluted samples are analyzed in 96-well plate for IFN-α protein concentration by VerikineTM Human IFN-α ELISA kit (#41100 R & D Systems). ..



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    Pathway analysis of key genes being up- or down-regulated on the transcript level upon 48 h of chemerin (huChem-157) treatment in OVCAR-3 cells as assessed by Affymetrix Clariom S human microarrays. Most of the up-regulated genes (red) are <t>IFNα-response</t> genes, with IRF9 as the key mediator of IFNα-induced gene regulation (as part of the ISGF3 complex, not shown). The observed regulation pattern is predicted to lead to growth inhibition of tumor cells (Ingenuity pathway analysis (IPA) software, Qiagen) (see discussion section). Broken arrows indicate transcriptional activation, other arrows protein interaction. The color intensity represents the measured grade <t>of</t> <t>mRNA</t> up- (red) or down-regulation (green).
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    Pathway analysis of key genes being up- or down-regulated on the transcript level upon 48 h of chemerin (huChem-157) treatment in OVCAR-3 cells as assessed by Affymetrix Clariom S human microarrays. Most of the up-regulated genes (red) are <t>IFNα-response</t> genes, with IRF9 as the key mediator of IFNα-induced gene regulation (as part of the ISGF3 complex, not shown). The observed regulation pattern is predicted to lead to growth inhibition of tumor cells (Ingenuity pathway analysis (IPA) software, Qiagen) (see discussion section). Broken arrows indicate transcriptional activation, other arrows protein interaction. The color intensity represents the measured grade <t>of</t> <t>mRNA</t> up- (red) or down-regulation (green).
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    Pathway analysis of key genes being up- or down-regulated on the transcript level upon 48 h of chemerin (huChem-157) treatment in OVCAR-3 cells as assessed by Affymetrix Clariom S human microarrays. Most of the up-regulated genes (red) are IFNα-response genes, with IRF9 as the key mediator of IFNα-induced gene regulation (as part of the ISGF3 complex, not shown). The observed regulation pattern is predicted to lead to growth inhibition of tumor cells (Ingenuity pathway analysis (IPA) software, Qiagen) (see discussion section). Broken arrows indicate transcriptional activation, other arrows protein interaction. The color intensity represents the measured grade of mRNA up- (red) or down-regulation (green).

    Journal: Cancers

    Article Title: Anti-Tumoral Effect of Chemerin on Ovarian Cancer Cell Lines Mediated by Activation of Interferon Alpha Response

    doi: 10.3390/cancers14174108

    Figure Lengend Snippet: Pathway analysis of key genes being up- or down-regulated on the transcript level upon 48 h of chemerin (huChem-157) treatment in OVCAR-3 cells as assessed by Affymetrix Clariom S human microarrays. Most of the up-regulated genes (red) are IFNα-response genes, with IRF9 as the key mediator of IFNα-induced gene regulation (as part of the ISGF3 complex, not shown). The observed regulation pattern is predicted to lead to growth inhibition of tumor cells (Ingenuity pathway analysis (IPA) software, Qiagen) (see discussion section). Broken arrows indicate transcriptional activation, other arrows protein interaction. The color intensity represents the measured grade of mRNA up- (red) or down-regulation (green).

    Article Snippet: Since pathway analyses strongly suggested IFNα to be the activator of most genes regulated after chemerin treatment in all cell lines tested, but we did not observe any induction of IFNα on the mRNA level itself, we tested the effect of this adipokine on the IFNα protein concentrations in cell culture supernatants using ELISA (DFNAS0, R&D Systems).

    Techniques: Inhibition, Software, Activation Assay

    Network of genes regulated after 48 h of chemerin (huChem-157)-treatment in at least two ovarian cancer cell lines, and connection to tumor growth inhibition. Activation of the network of interferon response genes is mediated by IFNα-induced formation of the upstream regulator and transcription factor ISGF3 (ISGF3 target genes are indicated by an asterisk). Broken arrows indicate transcriptional activation, other arrows protein interaction. Connection of genes to growth inhibition is indicated using solid arrows. The color intensity represents the measured grade of mRNA up- (red) or –down-regulation (green). * Type I interferon response genes (Ingenuity pathway analysis (IPA) software, Qiagen).

    Journal: Cancers

    Article Title: Anti-Tumoral Effect of Chemerin on Ovarian Cancer Cell Lines Mediated by Activation of Interferon Alpha Response

    doi: 10.3390/cancers14174108

    Figure Lengend Snippet: Network of genes regulated after 48 h of chemerin (huChem-157)-treatment in at least two ovarian cancer cell lines, and connection to tumor growth inhibition. Activation of the network of interferon response genes is mediated by IFNα-induced formation of the upstream regulator and transcription factor ISGF3 (ISGF3 target genes are indicated by an asterisk). Broken arrows indicate transcriptional activation, other arrows protein interaction. Connection of genes to growth inhibition is indicated using solid arrows. The color intensity represents the measured grade of mRNA up- (red) or –down-regulation (green). * Type I interferon response genes (Ingenuity pathway analysis (IPA) software, Qiagen).

    Article Snippet: Since pathway analyses strongly suggested IFNα to be the activator of most genes regulated after chemerin treatment in all cell lines tested, but we did not observe any induction of IFNα on the mRNA level itself, we tested the effect of this adipokine on the IFNα protein concentrations in cell culture supernatants using ELISA (DFNAS0, R&D Systems).

    Techniques: Inhibition, Activation Assay, Software

    Interferon alpha (IFNα) concentration (ng/mL) in cell culture supernatants of the indicated cell lines after treatment with huChem-157 (400 ng/mL) for 24 or 48 h as assessed via ELISA. * p < 0.05; ** p < 0.01.

    Journal: Cancers

    Article Title: Anti-Tumoral Effect of Chemerin on Ovarian Cancer Cell Lines Mediated by Activation of Interferon Alpha Response

    doi: 10.3390/cancers14174108

    Figure Lengend Snippet: Interferon alpha (IFNα) concentration (ng/mL) in cell culture supernatants of the indicated cell lines after treatment with huChem-157 (400 ng/mL) for 24 or 48 h as assessed via ELISA. * p < 0.05; ** p < 0.01.

    Article Snippet: Since pathway analyses strongly suggested IFNα to be the activator of most genes regulated after chemerin treatment in all cell lines tested, but we did not observe any induction of IFNα on the mRNA level itself, we tested the effect of this adipokine on the IFNα protein concentrations in cell culture supernatants using ELISA (DFNAS0, R&D Systems).

    Techniques: Concentration Assay, Cell Culture, Enzyme-linked Immunosorbent Assay